Some useful numbers such as surface area and volumes of dissociation solutions are given below for various size culture vessels.
6 well tissue culture plate surface area.
There are various sizes of dishes and flasks used for cell culture.
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Dish diameter actual area calculated area relative to 35 mm dish 35 mm 10 cm2 9 6 cm2 1 60 mm 220 cm 28 cm2 2 100 mm 60 cm 2 78 5 cm 6.
Growth area of tissue culture plates and dishes growth area cm 2.
1 4 cm2 well volume.
0 3 x 10 6.
Chart showing surface area seeding density cells at confluency.
1 2 x 10 6.
Cell culture plate multi well plates multiple well plates multiwell plates tissue culture plates.
48 well 11 0 0 95 9 5 x 104 1 6 0 19 0 285 transwell permeable supports transwell transwell insert approx.
Growth average insert format diameter mm area cm2 cell yield well insert 6 well 24 mm 4 67 cm2 4 67 x 105 2 6 1 5 12 well 12 mm 1 12 cm2 1 12 x 105 1 5 0 5 24 well 6 5 mm 0 33 cm2 3 3 x 104 0 6 0 1.
Corning costar tc treated multiple well plates size 6 wells clear polystyrene plate flat bottom case of 50 individually wrapped sterile lid.